Terminal deoxynucleotidyl transferase

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deoxynucleotidyltransferase, terminal
Identifiers
Symbol	DNTT
Entrez	1791
HUGO	2983
OMIM	187410
RefSeq	NM_004088
UniProt	P04053
Other data
EC number	2.7.7.31
Locus	Chr. 10 q23-q24

Terminal Deoxynucleotidyl Transferase, also known as TdT and terminal transferase, is a specialized DNA polymerase expressed in immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic leukemia/lymphoma cells. TdT adds N-nucleotides to the variable, diversity, and joining exons during antibody gene recombination.

TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a proturbing 3' overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.

Terminal transferase has applications in molecular biology. It can be used in RACE to add nucleotides which can then be used as a template for a primer in subsequent PCR. It can also be used to add nucleotides labelled with radioactive isotopes.

Also used in the immunofluorescence assay for the diagnosis of acute lymphoblastic leukemia.

It is the main enzym used in TUNEL assay (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) for the demonstration of apoptosis.

See also

• DNA
• DNA polymerase

External links

• MeSH Terminal+Deoxyribonucleotidyltransferase

Template:Enzyme-stub

v • d • e Phosphotransferases/kinases (EC 2.7)
2.7.1 - OH acceptor	Hexo- - Gluco- - Fructo- (Hepatic fructo-) - Galacto- - Phosphofructo- (1, 2) - Thymidine - NAD+ - Glycerol - Pantothenate - Mevalonate - Pyruvate - Deoxycytidine - PFP - Diacylglycerol - Bruton's tyrosine - Phosphoinositide 3 (Class I PI 3, Class II PI 3) - Sphingosine
2.7.2 - COOH acceptor	Phosphoglycerate - Aspartate
2.7.3 - N acceptor	Creatine
2.7.4 - PO4 acceptor	Phosphomevalonate - Adenylate - Nucleoside-diphosphate
2.7.6 - P2O7	Ribose-phosphate diphosphokinase - Thiamine pyrophosphokinase
2.7.7 - nucleotidyl-	Integrase - PNPase - Polymerase - RNase PH - UDP-glucose pyrophosphorylase - Galactose-1-phosphate uridylyltransferase -Terminal deoxynucleotidyl transferase - RNA replicase - Reverse transcriptase (Telomerase) - Transposase
2.7.8 - other phos.	N-acetylglucosamine-1-phosphate transferase
2.7.10-11 - protein	Tyrosine - Serine/threonine-specific