Colorimeter
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| Colorimeter | |
|---|---|
| Image:Spectrophoto-controls.svg | |
| Other Names | Colourimeter |
| Uses | Concentration measurement, display calibration |
| Inventor | Jan Szczepanik |
| Related | Spectrophotometer |
A colorimeter, an instrument used in colorimetry, can refer to one of several related devices. In scientific fields the word generally refers to the device that measures the absorbance of particular wavelengths of light by a specific solution. This device, invented by Jan Szczepanik, is most commonly used to determine the concentration of a known solute in a given solution by the application of the Beer-Lambert law, which states that the concentration of a solute is proportional to the absorbance. Other devices, also called colorimeters, are used in graphic design to calibrate display devices and printers.
Contents |
Construction
The essential parts of a colorimeter are:
- a light source (often an ordinary low-voltage filament lamp)
- an adjustable aperture
- a set of colored filters
- a cuvette to hold the working solution
- a detector (usually a photoresistor) to measure the transmitted light
- a meter to display the output from the detector
In addition, there may be:
- a voltage regulator, to protect the instrument from fluctuations in mains voltage.
- a second light path, cuvette and detector. This enables comparison between the working solution and a "blank", consisting of pure solvent, to improve accuracy.
Filters
Changeable optics filters are used in the colorimeter to select the wavelength of light which the solute absorbs the most, in order to maximize accuracy. The usual wavelength range is from 400 to 700 nanometres (nm). If it is necessary to operate in the ultraviolet range (below 400 nm) then some modifications to the colorimeter are needed. In modern colorimeters the filament lamp and filters may be replaced by several light-emitting diodes of different colors.
Cuvettes
- Main article: Cuvette
In a manual colorimeter the cuvettes are inserted and removed by hand. An automated colorimeter (as used in an AutoAnalyzer) is fitted with a flowcell through which solution flows continuously.
Controls
(1) Wavelength selection; (2) Printer button; (3) Concentration factor adjustement; (4) UV mode selector (Deuterium lamp); (5) Readout; (6) Sample compartement; (7) Zero control (100% T); (8) Sensitivity switch.
Output
The output from a colorimeter may be displayed by an analogue or digital meter and may be shown as transmittance (a linear scale from 0-100%) or as absorbance (a logarithmic scale from zero to infinity). The useful range of the absorbance scale is from 0-2 but it is desirable to keep within the range 0-1 because, above 1, the results become unreliable due to scattering of light.
In addition, the output may be sent to a chart recorder, data logger, or computer.
Graphic design
In graphic design, colorimeters are used to generate color profiles for equipment in the workflow. Accurate color profiles are important to ensure that screen displays match the final printed products.
See also
External links
Laboratory Equipment | |
|---|---|
| Equipment | Agar plate • Aspirator • Autoclave • Bunsen burner • Calorimeter • Colony counter • Colorimeter • Laboratory centrifuge • Fume hood • Glove box • Incubator • Homogenizer • Laminar flow cabinet • Magnetic stirrer • Microscope • Microtiter plate • Plate reader • Spectrophotometer • Stir bar • Thermometer • Vortex mixer • Static mixer |
| Glassware | Beaker • Boiling tube • Büchner funnel • Burette • Cold finger • Condenser • Conical measure • Crucible • Cuvette • Laboratory flasks (Erlenmeyer flask, Round-bottom flask, Florence flask, Volumetric flask, Büchner flask, Retort) • Gas syringe • Graduated cylinder • Glass tube • NMR tube • Pipette • Petri dish • Separating funnel • Soxhlet extractor • Test tube • Thistle tube • Watch glass |
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