CENPH

Centromere protein H
Identifiers
Symbol(s)	CENPH; PMF1; NNF1
External IDs	OMIM: 605607 MGI: 1349448 Homologene: 32519
Gene ontology Molecular Function: • protein binding • kinetochore binding Cellular Component: • kinetochore • nucleus • chromosome Biological Process: • kinetochore organization and biogenesis
Orthologs
	Human	Mouse
Entrez	64946	26886
Ensembl	ENSG00000153044	ENSMUSG00000045273
Uniprot	Q9H3R5	Q9QYM8
Refseq	NM_022909 (mRNA) NP_075060 (protein)	NM_021886 (mRNA) NP_068686 (protein)
Location	Chr 5: 68.52 - 68.54 Mb	Chr 13: 101.86 - 101.88 Mb
Pubmed search	[1]	[2]

Centromere protein H, also known as CENPH, is a human gene.

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.

Further reading

• Sugata N, Li S, Earnshaw WC, et al. (2001). "Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere--kinetochore complexes.". Hum. Mol. Genet. 9 (19): 2919-26. PMID 11092768.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-903. doi:10.1073/pnas.242603899. PMID 12477932.
• Saffery R, Sumer H, Hassan S, et al. (2003). "Transcription within a functional human centromere.". Mol. Cell 12 (2): 509-16. PMID 14536089.
• Obuse C, Yang H, Nozaki N, et al. (2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase.". Genes Cells 9 (2): 105-20. PMID 15009096.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121-7. doi:10.1101/gr.2596504. PMID 15489334.
• Obuse C, Iwasaki O, Kiyomitsu T, et al. (2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1.". Nat. Cell Biol. 6 (11): 1135-41. doi:10.1038/ncb1187. PMID 15502821.
• Mikami Y, Hori T, Kimura H, Fukagawa T (2005). "The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis.". Mol. Cell. Biol. 25 (5): 1958-70. doi:10.1128/MCB.25.5.1958-1970.2005. PMID 15713649.
• Tomonaga T, Matsushita K, Ishibashi M, et al. (2005). "Centromere protein H is up-regulated in primary human colorectal cancer and its overexpression induces aneuploidy.". Cancer Res. 65 (11): 4683-9. doi:10.1158/0008-5472.CAN-04-3613. PMID 15930286.
• Foltz DR, Jansen LE, Black BE, et al. (2006). "The human CENP-A centromeric nucleosome-associated complex.". Nat. Cell Biol. 8 (5): 458-69. doi:10.1038/ncb1397. PMID 16622419.
• Okada M, Cheeseman IM, Hori T, et al. (2006). "The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres.". Nat. Cell Biol. 8 (5): 446-57. doi:10.1038/ncb1396. PMID 16622420.
• Izuta H, Ikeno M, Suzuki N, et al. (2006). "Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells.". Genes Cells 11 (6): 673-84. doi:10.1111/j.1365-2443.2006.00969.x. PMID 16716197.
• Orthaus S, Ohndorf S, Diekmann S (2006). "RNAi knockdown of human kinetochore protein CENP-H.". Biochem. Biophys. Res. Commun. 348 (1): 36-46. doi:10.1016/j.bbrc.2006.06.187. PMID 16875666.
• Liao WT, Song LB, Zhang HZ, et al. (2007). "Centromere protein H is a novel prognostic marker for nasopharyngeal carcinoma progression and overall patient survival.". Clin. Cancer Res. 13 (2 Pt 1): 508-14. doi:10.1158/1078-0432.CCR-06-1512. PMID 17255272.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry.". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134. PMID 17353931.