BglII

File:BglII cut sites.JPG

BglII recognition site

BglII (pronounced "bagel two") is a type II restriction enzyme isolated from certain strains of Bacillus globigii and is part of the restriction modification system.

In molecular biology, it is a commonly used restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is A|GATCT, which is a palindrome as the complementary sequence is TCTAG|A.

Uses

Because of their ability to cut DNA in predictable locations and leave ends which can be ligated back together, restriction enzymes are commonly used in cloning, DNA screening, deletion mutagenesis, and many other commonly used techniques. Some of these enzymes produce sticky ends capable of base-pairing with the products of different restriction enzymes.

See also

• EcoRI, a nuclease enzyme from 'E. coli.
• FokI, a nuclease enzyme from Flavobacterium okeanokoites

References

1. Pingoud, A., Jeltsch, A. (2001) Structure and function of type II restriction endonucleases, Nucl. Acids Res. 29. 18, 3705-3727.

External links

• Restriction Enzyme DatabaseTemplate:Enzyme-stub